Patent · US Active

Microarray synthesis and assembly of gene-length polynucleotides

US8058004B2 · kind B2 · utility

103Cited by
105References
22Claims
0Family size

Assignee

Inventor

Key dates

Filing dateJun 22, 2009
Grant dateNov 15, 2011
Priority date—
Expiry dateJun 22, 2029

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC40B40/06
  • WIPO fieldOrganic fine chemistry
  • WIPO sectorChemistry

Abstract

There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.