Affinity ligands

US8058410B2 · kind B2 · utility

Assignees

• Sandoz AG · CH
• BOEHRINGER INGELHEIM RCV GMBH & CO KG · AT

Inventors

• Alois Jungbauer · Wien, AT
• Rainer Hahn · Tübingen, DE
• Waltraud Kaar · Wien, AT
• Michael Seifert · Wien, AT
• Bernhard Auer · Innsbruck, AT
• Clemens Achmüller · Kundl, AT
• Philipp Wechner · Innsbruck, AT

Key dates

• Filing date: Apr 25, 2006
• Grant date: Nov 15, 2011
• Expiry date: Dec 28, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/62
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed is an affinity matrix comprising a solid phase and an affinity ligand comprising peptide bonds coupled to this solid phase, wherein the affinity ligand comprising peptide bond is selected from the following group of ligands: a) peptides comprising the formula X1X2X3X4, wherein X1 to X4 are amino acid residues and at least two of X1 to X4 is W, Y or F; b) peptides comprising the formula X5X6X7X8, wherein X5 to X8 are amino acid residues, at least one of X5 to X8 is W, and at least one of X5 to X8 is E or D; and c) poly-amino acids consisting of an amino acid monomer of the group consisting of R, K, E and D and an amino acid monomer of the group consisting of Y, F and W, preferably poly-KY, poly-KF, poly-KW, poly-RY, poly-RF, poly-RW, poly-EY, poly-DY, poly-EF, poly-EW, poly-DF and poly-DW, with the proviso that the peptides according to a) and b) have a maximum length of 35 amino acid residues and that the poly-amino acids according to c) have a minimum length of 20 amino acid residues.