Site-targeted transformation using amplification vectors

US8058506B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE
• Sylvestre Marillonnet · Bodenwerder, DE

Key dates

• Filing date: Mar 22, 2002
• Grant date: Nov 15, 2011
• Expiry date: Apr 20, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8203
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process of causing a targeted integration of DNA of interest into a plant cell nuclear genome, comprising; i) providing plant cells with an amplification vector, or a precursor thereof, capable of autonomous replication in plant cells, said vector comprising; a) DNA sequence(s) encoding an origin of replication functional in plant cells, b) DNA sequence(s) necessary for site-specific and/or homologous recombination between the vector and a host nuclear DNA, and c) optionally, further DNA of interest; ii) optionally providing conditions that facilitate vector amplification and/or cell to cell movement and/or site-specific and/or homologous recombination, and iii) selecting cells having undergone recombination at a predetermined site in the plant nuclear DNA.