Method for making a polymer blend for reducing protein binding to surfaces

US8062881B2 · kind B2 · utility

Assignee

• CORNING INCORPORATED · US

Inventors

• Dana Craig Bookbinder · Corning, US
• Edward John Fewkes · Horseheads, US
• James Arthur Griffin · Corning, US
• Frances M. Smith · Elmira, US
• David Lambie Tennent · Corning, US

Key dates

• Filing date: Jul 8, 2010
• Grant date: Nov 22, 2011
• Expiry date: Jul 8, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C08J2471/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Hydrophobic polymer surfaces whose level of protein binding is less than about 50-80 ng/cm2 are achieved by: (1) applying a coating solution composed of a solvent and a non-ionic surfactant having a HLB number of less than 5 to the surface; and (2) drying the surface to remove the solvent and thereby bring the surfactant into direct contact with the hydrophobic polymer. The combination of a low HLB number and the drying step have been found to produce low binding surfaces which can withstand multiple washes with water and/or protein-containing solutions Alternatively, the low binding surfaces can be produced by applying the non-ionic surfactant to the mold surfaces which contact molten polymer and form the polymer into a desired shape, e.g., into a multi-well plate, a pipette tip, or the like. Further, the low binding surfaces may be produced by incorporating non-soluble, non-ionic surfactants having an HLB number of less than or equal to 10 into a polymer blend prior to molding the article.