Diagnostic histopathology using multiplex gene expression FISH
US8062897B2 · kind B2 · utility
5Cited by
12References
22Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 14, 2006 |
| Grant date | Nov 22, 2011 |
| Priority date | — |
| Expiry date | Dec 4, 2026 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method for detecting one or more nascent RNAs in a tissue sample using FISH. In the method, a plurality of probes (8 to 82) may be used to detect a single species of nascent RNA. Further, a plurality of nascent RNA species may be detected simultaneously using between 8 and 82 probes for each nascent RNA. The invention comprises, in addition, methods of preparing a sample for nascent RNA detection by reducing autofluorescence of the tissue sample. These techniques may be synergistically combined to achieve significantly improved results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.