Binding method and apparatus for sorting objects
US8067170B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 11, 2008 |
| Grant date | Nov 29, 2011 |
| Priority date | — |
| Expiry date | Sep 11, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/367
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method and apparatus of sorting objects including, providing a sample having wanted objects and unwanted objects; coating a surface of a sample holder with an antibody; placing an eluted sample on the sample holder; binding an antigen in the wanted objects with the antibody on the surface of the sample holder to sort the objects into wanted objects and unwanted objects; separating the wanted objects; and performing PCR-based STR analysis on the wanted objects. In one embodiment, holographic optical trapping is used to further sort the wanted objects. In other embodiments, the wanted objects are sperm and the antibody is a human sperm specific antibody, and the PCR is single cell PCR-based STR analysis. In still other embodiments, the binding is direct or indirect, ligands are used to bind to object-specific organomolecules, and protein A or protein G are used to bind the antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.