6-O sulfated polysaccharides and methods of preparation thereof
US8067196B2 · kind B2 · utility
3Cited by
3References
25Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 28, 2002 |
| Grant date | Nov 29, 2011 |
| Priority date | — |
| Expiry date | Oct 25, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2510/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods of 6-O-sulfating glucosaminyl N-acetylglucosamine residues (GlcNAc) in a polysaccharide preparation and methods of converting anticoagulant-inactive heparan sulfate to anticoagulant-active heparan sulfate and substantially pure polysaccharide preparations may by such methods. Also disclosed is a mutant CHO cell which hyper-produces anticoagulant-active heparan sulfate. Methods for elucidating the sequence of activity of enzymes in a biosynthetic pathway are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.