Fusion proteins having mutated immunoglobulin hinge region

US8067548B2 · kind B2 · utility

Assignee

• NOVAGEN HOLDING CORPORATION · KY

Inventors

• Haitao Wang · Nanhu, CN
• Yong Du · Shanghai, CN
• Rui Zhang · Plainsboro, US
• Jing Xu · Beijing, CN
• Longbin Liu · Thousand Oaks, US

Key dates

• Filing date: Jul 25, 2008
• Grant date: Nov 29, 2011
• Expiry date: Jul 25, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A fusion protein having a non-immunoglobulin polypeptide having a cysteine residue proximal to the C terminal thereof, and an immunoglobulin component with a mutated hinge region is provided. The mutation comprises a point mutated site corresponding in position to the position in a native hinge region of the cysteine residue located nearest the cysteine residue of the non-Ig component. The distance from the cysteine residue of the non-immunoglobulin polypeptide and any remaining cysteine residues of the mutated hinge region is sufficient to prevent the formation of a disulphide bond therebetween.