System with extended range of molecular sensing through integrated multi-modal data acquisition

US8072585B2 · kind B2 · utility

Assignee

• Purdue Research Foundation · US

Inventors

• Xuefeng Wang · Lo Wu, CN
• David D. Nolte · Lafayette, US
• Manoj Varma · West Lafayette, US
• Brian Weichel · Lafayette, US
• Timothy Norwood · Cambridge, GB
• Fouad Sayegh · West Lafayette, US
• Ming Zhao · Beijing, CN

Key dates

• Filing date: Dec 31, 2009
• Grant date: Dec 6, 2011
• Expiry date: Dec 31, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/253
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A multi-modal data acquisition system for detecting target material on a biological reaction surface, the system comprising a radiation source for generating an incoming beam that impinges on the biological reaction surface at an oblique incidence angle and produces a reflected beam, an interferometric detector for detecting an interferometric signal from the illuminated surface, the reflected beam being directed to the interferometric detector, a fluorescence detector for detecting a fluorescence signal from the illuminated surface; the fluorescence detector being positioned to substantially minimize the incidence of the reflected beam; and a processing system for receiving the interferometric and fluorescence signals and determining the presence or absence of target material on the biological reaction surface. A reaction surface conditioned for the simultaneous collection of fluorescence, interferometric and other signals. A multi-modal data acquisition system for collecting and processing additional modes, including multiple interferometric, fluorescence and scattering channels.