Polymeric sorbent sheets as ion reservoirs for electroblotting
US8075755B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Dec 13, 2007 |
| Grant date | Dec 13, 2011 |
| Priority date | — |
| Expiry date | Nov 16, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/26
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Electroblotting for the transfer of electrophoretically separated species from a gel to a transfer membrane is performed in a semi-dry format with sheets of absorbent polyester or polyester/cellulose blend wetted with buffer solution in place of the traditional buffer-wetted filter paper. The result is effective electroblotting at a lower electric current level than that obtained with filter paper and thereby less resistance heating of the gel, the transfer membrane, and the species being transferred.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.