Method for the treatment of a sample containing biomolecules

US8076069B2 · kind B2 · utility

Assignee

• QIAGEN GmbH · DE

Inventors

• Christian Korfhage · Hilden, DE
• Friederike Wilmer · Essen, DE
• Dirk Löffert · Mülheim an der Ruhr, DE
• Ralf Himmelreich · Langenfeld, DE
• Claudia Fritz
• Kathleen Rieske · Burscheid, DE

Key dates

• Filing date: Jun 3, 2009
• Grant date: Dec 13, 2011
• Expiry date: Jun 12, 2029

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/6803
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention generally provides a method for the sample preparation for a subsequent preparation, processing or analysis method of a sample containing an at least one species of nucleic acid and/or one species of protein, whereby the method comprises the following steps: A) providing a sample which contains at least one species of a nucleic acid and/or of a protein, B) contacting the sample with a fluid or solid composition to produce a fluid sample preparation, whereby the composition contains at least a nitrogenous compound, which is selected from the group consisting of a) polyamines, b) amino acids, and oligo- and polypeptides, c) nitrogenous heterocyclic compounds, including homo- oder heteropolymers, which comprise these nitrogenous compounds, d) amines of the type R1R2NR3, whereby R1, R2 and R3 are chosen independently from one another from the group consisting of H, C1-C5-alkyl groups and aryl groups, whereby R1, R2 and R3 are not H simultaneously, e) carboxylic acid amides, f) inorganic ammonium salts, g) ammonium groups containing inner salt compounds, h) antibiotica binding nucleic acid, i) compounds which bind in the small cavity of the DNA, and mixtures of two or more…