Methods for production of oligonucleotides
US8080393B2 · kind B2 · utility
12Cited by
31References
41Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 10, 2006 |
| Grant date | Dec 20, 2011 |
| Priority date | — |
| Expiry date | Mar 7, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.