Patent · US Active

Alpha-amylase variants having an elevated solvent stability, method for the production thereof and detergents and cleansers containing these alpha-amylase variants

US8080401B2 · kind B2 · utility

5Cited by

9References

15Claims

0Family size

Assignee

Henkel AG & Co. KGaA · DE

Inventors

Cornelius Bessler · Mülheim an der Ruhr, DE
Susanne Wieland · Nievenheim, DE
Karl-Heinz Maurer · Erkrath, DE

Key dates

Filing date	Mar 28, 2007
Grant date	Dec 20, 2011
Priority date	—
Expiry date	Oct 14, 2028

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N9/2417
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention relates to α-amylase variants that are stabilized to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, by point mutagenesis of asparagine (N) or glutamine (Q) residues located on the surface of the molecule to give other amino acid residues. The invention further relates to methods of increasing the stability of an α-amylase to solvent-exerted hydrolysis, in particular at elevated temperatures or high pH, whereby at least one asparagine (N) or glutamine (Q) residue on the surface of the molecule is replaced with a different amino acid residue. The α-amylase variants obtained thereby exhibit better stability to influences of the solvent, increased processivity, and are suited for numerous industrial areas of use, in particular as active ingredients in detergents and cleansers.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.