Method and apparatus for analyzing individual cells or particulates using fluorescent quenching and/or bleaching
US8081303B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 20, 2009 |
| Grant date | Dec 20, 2011 |
| Priority date | — |
| Expiry date | Apr 14, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/062
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for analyzing a blood sample is provided that includes the steps of: a) providing a blood sample having one or more first constituents and one or more second constituents, which second constituents are different from the first constituents; b) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by a first panel and a second panel, both of which panels are transparent; c) admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce upon exposure to predetermined first wavelengths of light, and which colorant is operative to absorb light at one or more predetermined second wavelengths of light; d) illuminating at least a portion of the sample containing the first constituents and the second constituents at the first wavelengths and at the second wavelengths; e) imaging the at least a portion of the sample, including producing image signals indicative of fluorescent emissions from the first constituents and the second constituents and the optical density of the first constituents and the second constituents; f) determining a fluorescence…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.