Multimarking fibre-type fluorescence microscopic imaging method and system
US8081310B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jun 9, 2005 |
| Grant date | Dec 20, 2011 |
| Priority date | — |
| Expiry date | Feb 18, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6423
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method for producing a fluorescent fiber image of a sample, wherein a sample is scanned with the aid of an excitation signal; the fluorescent signal emanating from the sample is detected, wherein the excitation signal and fluorescent signal use the same optical path; the optical path is used to excite at least two fluorophores contained in the sample; a final image is produced, including areas that are colored according to the at least two fluorophores. The multimarking according to the invention makes it possible to simultaneously acquire two images in two different wavelength bands. The system according to the invention can comprise a spectrometer for spectral quantification of the fluorescent signal.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.