Treatment solution, method for pretreatment, method for acquiring information and method for detection
US8084214B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 7, 2008 |
| Grant date | Dec 27, 2011 |
| Priority date | — |
| Expiry date | May 30, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54313
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.