Method for glucose monitoring using fluorescence quenching

US8088595B2 · kind B2 · utility

Assignee

• THE TEXAS A&M UNIVERSITY SYSTEM · US

Inventors

• Bennett L. Ibey · San Antonio, US
• Vamsi K. Yadavalli · Richmond, US
• Rebecca M. Rounds · College Station, US
• Hope T. Beier · College Station, US
• Gerard L. Coté · College Station, US
• Michael V. Pishko · Austin, US

Key dates

• Filing date: Mar 5, 2010
• Grant date: Jan 3, 2012
• Expiry date: Apr 29, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/54366
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This disclosure relates to a method of measuring a glucose concentration metric or a glucose metric in a patient by contacting an implantable glucose-sensing device with a test sample, which may be in the patient, under conditions that permit a sugar-binding molecule and a functionalized polymer or nano-particle ligand present throughout the matrix of a hydrogel to interact in a glucose-dependent manner to produce an optical signal resulting from quenching of a first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenched upon binding or release of glucose. Next the first fluorophore may be excited with light of a certain wavelength. Then at least one wavelength of light in the glucose-dependent optical signal from the fluorophore may be detected with a detector to produce a detected light signal, which may be processed to produce a glucose metric, such as a glucose concentration metric.