Nucleic acids encoding genetically modified tissue factor pathway inhibitor (TFPI) and method of making the same

US8088599B2 · kind B2 · utility

Assignee

• FUDAN UNIVERSITY · CN

Inventors

• Duan Ma · Shanghai, CN
• Jingui Mu · Shanghai, CN
• Jiping Wang · Sharonville, US
• Huijun Wang · Beijing, CN
• Wang Liang · Shanghai, CN

Key dates

• Filing date: Mar 19, 2009
• Grant date: Jan 3, 2012
• Expiry date: Nov 27, 2029

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61P7/02
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides long half life genetically modified TFPI sequences (LTFPI) for anticoagulation. On the genetically modified TFPI sequence, the lysine at the carboxy-terminal sites 241, 254, 260 and 261 are replaced by alanin and the amino acid asparagine at glycosylation sites 117, 167, 228 and the amino acids serine and threonine at glycosylation sites 174 and 175 are substitutionally mutated. The present invention also provides methods of making the LTFPI through high efficient LTFPI expression from yeast production system.