Expression systems for functional membrane polypeptides
US8088601B2 · kind B2 · utility
5Cited by
5References
22Claims
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Key dates
| Filing date | Nov 10, 2008 |
| Grant date | Jan 3, 2012 |
| Priority date | — |
| Expiry date | Oct 1, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Expression systems and methods for the expression of functional membrane polypeptides such as human cytochrome b5 are provided. The systems include recombinant expression vectors capable of expressing soluble fusion proteins that include a solubilizing agent, a linker, and a membrane polypeptide, as well as one or more cleavers, e.g. proteases, capable of cleaving the linker to release the membrane polypeptide. When the fusion protein is expressed, the linker is cleaved by the cleaver to allow association of the membrane polypeptide with a membrane.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.