Patent · US Active

Method for determining the viability of cells in cell cultures

US8093015B2 · kind B2 · utility

10Cited by

0References

13Claims

0Family size

Assignee

Sartorius Stedim Biotech GmbH · DE

Inventors

Stefan Obermann · Dransfeld, DE
Reinhard Baumfalk · Oettingen, DE
Oscar-Werner Reif · Hannover, DE
Florian M. Wurm · Prilly, CH
Maria De Jesus · Yverdon-les-Bains, CH
Matthieu Stettler · Vucherens, CH
Martin Jordan · Orpington, GB

Key dates

Filing date	Apr 24, 2007
Grant date	Jan 10, 2012
Priority date	—
Expiry date	Apr 30, 2028

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10T436/25375
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to a method for determining the viability of cells in cell cultures, said method comprising the steps of: coloring the sample with a dye which can penetrate into individual cells depending on the viability of the latter, determining the proportions of cells which are colored to differing extents. The invention is distinguished by virtue of the fact that a cell suspension of the colored cells is centrifuged as a sample in a sample vessel (10) until the solid content present in the sample has settled as a compressed cell cake (13), and, in order to determine the proportions, the volumes of differently colored sections (131, 132, 133) of the compressed cell cake (13) are determined as a measure of the proportions of cells of different viability.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.