Biologically safe transient protein expression in plants

US8093458B2 · kind B2 · utility

Assignee

• Icon Genetics GmbH · DE

Inventors

• Sylvestre Marillonnet · Bodenwerder, DE
• Carola Engler · Bodenwerder, DE
• Stefan Muhlbauer · Freising, DE
• Stefan Herz · Bocholt, DE
• Stefan Werner · Halle (Saale), DE
• Victor Klimyuk · Leipzig, DE
• Yuri Gleba · Berlin, DE

Key dates

• Filing date: Jul 7, 2005
• Grant date: Jan 10, 2012
• Expiry date: Apr 9, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8257
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A process of producing a protein of interest by expression of said protein of interest from a sequence of interest in a plant or in plant leaves, comprising: (a) transfecting said plant or said plant leaves by infiltrating said plant or said plant leaves with an Agrobacterium strain in the presence of a complementing factor, said Agrobacterium strain containing in T-DNA a heterologous DNA sequence having a sequence portion encoding a replicon, wherein said sequence encoding a replicon contains sequences necessary for replicon function of said replicon, said sequences being derived from a plant virus, and said sequence of interest to be expressed from said replicon, (b) optionally isolating said protein of interest from said plant or said plant leaves infiltrated in step (a), wherein said Agrobacterium strain is provided with a first genetic modification rendering said Agrobacterium strain defective for transfecting organisms with said T-DNA in the absence of said complementing factor.