Efficient optical arrangement for illumination and detection of label-free biosensors and method to reduce interference fringes in label-free imaging
US8094305B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 25, 2009 |
| Grant date | Jan 10, 2012 |
| Priority date | — |
| Expiry date | Jul 27, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N21/7743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An optical arrangement for illuminating a surface of a biosensor is described. The biosensor is preferably a sensor having periodic surface grating structure. The arrangement includes a light source generating light, collimating optics for collimating the light from the light source, and first reflecting surface receiving light from the collimating optics and directing incident light onto a surface of the biosensor and a second spatially separated reflecting surface receiving light reflected from the surface of the biosensor. The arrangement further includes telecentric optics (e.g., telecentric lens) receiving light from the second surface of the prism. The telecentric lens directs light onto an entrance slit of a spectrometer. The arrangement increases the light collection efficiency at the spectrometer as compared to prior art arrangements. The use of an incoherent light source and an arrangement in which incident light impinges upon the biosensor surface at a non-normal angle of incidence parallel to the direction of the grating lines on the sensor eliminates undesirable interference fringes in peak wavelength value measurements of the sensor.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.