Reversible electrochemical sensors for polyions
US8097135B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 27, 2006 |
| Grant date | Jan 17, 2012 |
| Priority date | — |
| Expiry date | Jul 1, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/327
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention is directed to a reversible electrochemical sensor for polyions. The sensor uses active extraction and ion stripping, which are controlled electrochemically. Spontaneous polyion extraction is suppressed by using membranes containing highly lipophilic electrolytes that possess no ion-exchange properties. Reversible extraction of polyions is induced by constant current pulse of fixed duration applied across the membrane. Subsequently, polyions are removed by applying a constant stripping potential. The sensors provide excellent stability and reversibility and allow for measurements of heparin concentration in whole blood samples via protamine titration. The sensors can also monitor a polyion concentration and an enzyme activity, wherein the polyion decomposition is directly proportional to the enzyme activity in a sample. Additionally, the sensors can monitor an enzyme inhibitor activity. Also, an immunoassay can be used to detect analytes by employing one of a polyion and an enzyme as markers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.