System and method for forming skeletal muscle constructs having functional tissue interfaces

US8097455B2 · kind B2 · utility

Assignee

• THE REGENTS OF THE UNIVERSITY OF MICHIGAN · US

Inventors

• Lisa M. Larkin · Ann Arbor, US
• Ellen M. Arruda · Ann Arbor, US
• Sarah Calve · West Lafayette, US
• Tatiana Y. Kostriminova · Schererville, US

Key dates

• Filing date: Feb 4, 2008
• Grant date: Jan 17, 2012
• Expiry date: Sep 5, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2533/52
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A system and method for forming a skeletal muscle construct include primary muscle cells provided on a substrate without disposing the cells within an exogenous scaffold, the cells cultured in vitro such that the cells form a confluent monolayer; at least two anchors secured to the monolayer in spaced relationship; and at least one secondary tissue, such as neural or tendon tissue, provided in contact with the monolayer such that the monolayer detaches from the substrate and self-organizes to at least partially surround the at least one secondary tissue, thereby forming a three-dimensional skeletal muscle construct having a functional interface with the secondary tissue.