Modified cellulases with increased thermostability, thermophilicity, and alkalophilicity
US8101398B2 · kind B2 · utility
5Cited by
3References
23Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 23, 2010 |
| Grant date | Jan 24, 2012 |
| Priority date | — |
| Expiry date | Jul 17, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01091
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A modified Family 6 cellulase enzyme comprising a proline residue at position 413 is provided. Genetic constructs and genetically modified microbes comprising DNA sequences encoding the modified Family 6 cellulase are also provided. Family 6 cellulases of the invention display improved thermostability, thermophilicity, alkalophilicity, or a combination thereof, relative to the parent Family 6 cellulases. Such cellulases find use in a variety of applications in industry that require cellulase stability and activities at temperatures, pH values, or both, above that of the native enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.