Methods of producing validamycin A analogs and uses thereof

US8101732B2 · kind B2 · utility

Assignee

• State of Oregon acting by and through the State Board of Higher Ed. on Behalf of Oregon State Univ. · US

Inventors

• Taifo Mahmud · Corvallis, US
• Zixin Deng · 红钢城街道, CN
• Linquan Bai · Shanghai, CN
• Hui Xu · Lo Wu, CN
• Jongtae Yang · Eugene, US

Key dates

• Filing date: Sep 15, 2009
• Grant date: Jan 24, 2012
• Expiry date: Oct 8, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P19/46
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This disclosure relates to validamycin A biosynthesis and in particular, to methods of producing validamycin A analogs and uses thereof. In a particular example, a method for making a validamycin A analog includes transforming a host cell with one or more recombinant DNA vectors to produce a valN-inactivated mutant; and culturing the valN-inactivated mutant in a culture medium to produce a validamycin A analog, such as 1,1′-bis-valienamine and validienamycin, and their conversion to valienamine. The present disclosure further relates to compositions including such compounds as well as methods of using the compositions, such as for antifungal agents.