Method and system for microfluidic device and imaging thereof

US8105550B2 · kind B2 · utility

Assignee

• FLUIDIGM CORPORATION · US

Inventors

• Emerson Chueng Quan · South San Francisco, US
• Colin Jon Taylor · Orinda, US
• Michael Lee · Lake Oswego, US
• Christopher G. Cesar · Sunnyvale, US
• Greg Harris · Opelika, US
• Gang Sun · Chesterfield, US

Key dates

• Filing date: Dec 22, 2009
• Grant date: Jan 31, 2012
• Expiry date: Jul 28, 2030

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/11
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A biological substrate, e.g., microfluidic chip. The substrate includes a rigid substrate material, which has a surface region capable of acting as a handle substrate. The substrate also has a deformable fluid layer coupled to the surface region. One or more well regions are formed in a first portion of the deformable fluid layer and are capable of holding a fluid therein. The one or more channel regions are formed in a second portion of the deformable fluid layer and are coupled to one or more of the well regions. An active region is formed in the deformable fluid layer. At least three fiducial markings are formed within the non-active region and disposed in a spatial manner associated with at least one of the well regions. A control layer is coupled to the fluid layer.