Patent · US Active

Aptamers and methods for their in vitro selection and uses thereof

US8105982B2 · kind B2 · utility

3Cited by

10References

8Claims

0Family size

Assignee

The Regents of the University of California · US

Inventors

Sharon A. Doyle · Walnut Creek, US
Michael Murphy · Rochester, US

Key dates

Filing date	Feb 6, 2008
Grant date	Jan 31, 2012
Priority date	—
Expiry date	Dec 26, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6811
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present method is an improved in vitro selection protocol that relies on magnetic separations for DNA aptamer production that is relatively easy and scalable without the need for expensive robotics. The ability of aptamers selected by this method to recognize and bind their target protein with high affinity and specificity, and detail their uses in a number of assays is also described. Specific TTF1 and His6 aptamers were selected using the method described, and shown to be useful for enzyme-linked assays, Western blots, and affinity purification.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.