PCR method for identifiying a serotype of Klebsiella pneumoniae by using cps region primers and application thereof
US8110359B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Apr 15, 2009 |
| Grant date | Feb 7, 2012 |
| Priority date | — |
| Expiry date | Mar 26, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method of identifying a serotype of Klebsiella pneumoniae, in particular to a method using specific polymerase chain reaction (PCR) primer sets designed according to a fragment of a capsular polysaccharide synthesis (cps) region to identify a K57 or a NTUH-N1 serotype and its application. NTUH-N1 is a novel serotype which differs from the previously reported 77 serotypes. This PCR-based cps genotyping method not only solves the problems of insufficient specificity and sensitivity caused by conventional immune method, but can be applied in clinical diagnosis with the advantages of rapidity and low cost. In addition, the rate of unidentifiable strains can also be reduced by this method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.