Method of producing biologically active polypeptide having insulinotropic activity

US8114632B2 · kind B2 · utility

Assignee

• Biocon Ltd. · IN

Inventors

• Ramakrishnan Melarkode · Savanuru, IN
• Akundi Venkata Sriram · Bengaluru, IN
• Kedarnath Sastry · Savanuru, IN
• Lakshmi Prabha Varadarajalu · Bengaluru, IN
• Shrikumar Suryanarayan · Savanuru, IN

Key dates

• Filing date: Jun 20, 2007
• Grant date: Feb 14, 2012
• Expiry date: May 29, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K14/46
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to a method of producing a biologically active polypeptide having insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified host cell that has protease gene knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed host cell to produce the biologically active polypeptide; and a method of producing a biologically active polypeptide having an N-terminal recognition site His-Gly with insulinotropic activity, the method comprising steps of: (a) transforming a genetically modified Pichia pastoris that has protease gene STE13 knockout, with a polynucleotide vector encoding the polypeptide; and (b) growing the transformed Pichia pastoris to produce the biologically active polypeptide.