Determination of the hydrodynamic radii of the constituents of an admixture by means of analysis of a taylor dispersion carried out following a separation by means of capillary electrophoresis
US8118986B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 14, 2007 |
| Grant date | Feb 21, 2012 |
| Priority date | — |
| Expiry date | Feb 29, 2028 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44726
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for determining the hydrodynamic radius for the constituents of an admixture, includes: (A) by separating capillary electrophoresis, the constituents of the admixture, leaving them within the capillary; (B) at one of the ends of the capillary obtained in this manner, containing, in different zones, the separated constituents, a detectable marker is injected in the region of a detection device which is placed at the side of the other end of the capillary; (C) a pressure difference is induced between the ends of the capillary in order to cause the various constituents separated in step (A) and finally the marker to migrate towards the outlet of the capillary; and (D) by analyzing the Taylor dispersion produced in step (C), the hydrodynamic radius is determined for each of the constituents, based on the detection time of the marker and the elution profile of each of the constituents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.