Patent · US Active

Rapid one-step reverse transcriptase PCR

US8119353B2 · kind B2 · utility

0Cited by

10References

7Claims

0Family size

Assignee

Roche Diagnostics Operations, Inc. · US

Inventors

Waltraud Ankenbauer · Penzberg, DE
Ursula Grepl · Gräfelfing, DE
Rita Haerteis · Mannheim, DE

Key dates

Filing date	Apr 3, 2008
Grant date	Feb 21, 2012
Priority date	—
Expiry date	Jan 2, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6846
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed to a method for performing a one-step RT-PCR for amplifying a target RNA comprising the steps (i) providing a sample which is supposed to contain said target RNA (ii) adding a reaction mixture comprising all reagents necessary to reverse transcribe said target RNA into cDNA and amplify at least a portion of said cDNA (iii) incubating said sample for a time interval of 0 seconds to 40 seconds at a temperature between 20° C. and 65° C., and (iv) subjecting said sample to multiple cycles of a thermocycling protocol wherein the temperature of said sample is varied between at least a first temperature between 37° C. and 72° C. and a second temperature between 85° C. and 100° C.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.