Purification of a bivalently active antibody using a non-chromatographic method
US8124743B2 · kind B2 · utility
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17Claims
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Key dates
| Filing date | Jun 1, 2007 |
| Grant date | Feb 28, 2012 |
| Priority date | — |
| Expiry date | Jan 22, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a method of purifying bivalent antibodies or antibody fragments that are active at both Fab sites from a source of antibodies or antibody fragments using a non-chromatographic method that includes inducing the formation of cyclic immunoglobulin aggregates by addition of multivalent hapten to a salt solution of soluble antibodies or antibody fragments, wherein the multivalent hapten possesses a linker between the two haptens effective to prevent the binding of both haptens of the ligand to the same antibody or antibody fragment.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.