Method of preparing 5′-amino-linker oligonucleotides derivatives and analogous 5′-labeled-linker oligonucleotides therefrom
US8124756B2 · kind B2 · utility
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Key dates
| Filing date | Sep 15, 2009 |
| Grant date | Feb 28, 2012 |
| Priority date | — |
| Expiry date | Oct 27, 2029 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for preparing amino linker oligonucleotides is provided. More specifically, a method of preparing 5′-amino-linker oligonucleotides comprising the steps of: introducing an amino linker having a protecting group into the 5′ terminus of an oligonucleotide; and removing the protecting group from the amino linker oligonucleotide by contacting with acetic acid and 2,2,2-trifluoroethanol is provided. The amino protecting group is efficiently removed from the amino linker oligonucleotides, and thereby achieving a high yield of the amino linker oligonucleotides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.