Minigene expression cassette

US8129510B2 · kind B2 · utility

Assignees

• The Board of Trustees of the Leland Stanford Junior University · US
• Benitec Biopharma Limited · AU

Inventors

• Mark A. Kay · Los Altos, US
• Micheal Hebert · Sunnyvale, US
• Petrus W. Roelvink · Campbell, US
• David Suhy · San Ramon, US

Key dates

• Filing date: Mar 30, 2007
• Grant date: Mar 6, 2012
• Expiry date: Mar 30, 2027

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2830/85
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods and compositions for expressing a gene or nucleotide sequence of interest are provided. The compositions include an expression cassette that includes a synthetic enhancer, a transthyretin promoter, and a nucleotide sequence operably under the control of the synthetic enhancer and the transthyretin promoter. The expression cassette may be used in an adeno-associated viral (AAV) vector, such as a self-complementary AAV vector.