Protoporphyrinogen oxidase having activity of imparting resistance against acifluorfen and gene thereof
US8129589B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Sep 25, 2006 |
| Grant date | Mar 6, 2012 |
| Priority date | — |
| Expiry date | Oct 13, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8274
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Protoporphyrinogen oxidase having an activity of imparting acifluorfen resistance and gene thereof are provided. Cyanobacterium protoporphyrinogen oxidase gene is identified by introducing a protoporphyrinogen oxidase gene of Arabidopsis into cyanobacterium, disrupting a cyanobacterium gene with a transposon, selecting a mutant strain in which protoporphyrinogen oxidase gene is disrupted, identifying the disrupted protoporphyrinogen oxidase gene, and isolating the disrupted protoporphyrinogen oxidase gene. This procedure is effective as a gene isolation technique when a protein derived from other organism species that is homologous to a known protein (e.g., protoporphyrinogen oxidase from cyanobacterium) can not be found in a gene database of the other species.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.