Chemically modified nucleoside 5′-triphosphates for thermally initiated amplification of nucleic acid

US8133669B2 · kind B2 · utility

Assignee

• TriLink BioTechnologies, LLC · US

Inventors

• Alexandre Lebedev · San Diego, US
• Inna KOUKHAREVA · San Diego, US

Key dates

• Filing date: May 21, 2009
• Grant date: Mar 13, 2012
• Expiry date: May 21, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and/or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and/or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.