Two slow-step polymerase enzyme systems and methods

US8133672B2 · kind B2 · utility

Assignee

• PACIFIC BIOSCIENCES OF CALIFORNIA, INC. · US

Inventors

• Keith Bjornson · Fremont, US
• Harold Lee · Holly Springs, US
• Jonas Korlach · Camas, US
• Stephen Turner · Menlo Park, US
• Arek Bibillo · Cupertino, US
• Fred Christians · Los Altos Hills, US
• Robin Emig · Belmont, US
• Molly He · San Diego, US
• Insil Park · Fremont, US
• Lei Jia · Palo Alto, US
• Andrei G. Fedorov · Atlanta, US
• John Lyle · Fremont, US

Key dates

• Filing date: Mar 30, 2009
• Grant date: Mar 13, 2012
• Expiry date: Oct 17, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Compositions, kits, methods and systems for nucleotide sequencing comprising producing polymerase reactions that exhibit two kinetically observable steps within an observable phase of the polymerase reaction. Two slow step systems can be produced, for example, by selecting the appropriate polymerase enzyme, polymerase reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.