Target sequences for synthetic molecules
US8133709B2 · kind B2 · utility
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14References
23Claims
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Assignee
Inventor
Key dates
| Filing date | Apr 3, 2008 |
| Grant date | Mar 13, 2012 |
| Priority date | — |
| Expiry date | Mar 29, 2030 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/532
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention is based on the discovery that certain biarsenical molecules react with specified target sequences, thereby providing a facile means for labeling polypeptides containing the target sequence. The invention is useful in creating stable mammalian cell lines expressing a certain tetracysteine tagged polypeptides, thereby overcoming toxicity associated with native tetracysteine. In addition, the invention allows for orthogonal labeling of polypeptides, thereby allowing for the observation of protein-protein interactions and conformational changes in proteins, for example.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.