Preparation and use of single-stranded transcription substrates for synthesis of transcription products corresponding to target sequences
US8137911B2 · kind B2 · utility
48Cited by
55References
24Claims
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Key dates
| Filing date | Nov 21, 2003 |
| Grant date | Mar 20, 2012 |
| Priority date | — |
| Expiry date | Jan 15, 2026 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1247
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods, compositions and kits for using an RNA polymerase for making transcription products corresponding to a target sequence by obtaining single-stranded DNA transcription substrates that comprise a single-stranded promoter that is operably joined to a single-stranded target sequence. The invention has broad applicability for research, diagnostic and therapeutic applications, such as preparing cDNA corresponding to full-length mRNA, making sense or anti-sense probes, detecting gene- or organism-specific sequences, cloning, cell signaling or making RNA for use in RNAi.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.