Feline pancreatic lipase

US8138316B2 · kind B2 · utility

Assignee

• IDEXX LABORATORIES INC. · US

Inventors

• Philip Andersen · Poland, US
• Melissa Beall · Cape Elizabeth, US
• Jesse Buch · Kennebunk, US
• Ku-chuan Hsiao · Easton, US
• Stacey Pazar Huth · Scarborough, US
• Eugene Regis Krah, III · Freeport, US
• Barbara O'Brien · Gorham, US
• Marilyn Strong-Townsend · Yarmouth, US

Key dates

• Filing date: Jun 10, 2010
• Grant date: Mar 20, 2012
• Expiry date: Jun 10, 2030

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K16/40
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Isolated nucleic acid molecules having a nucleotide sequence encoding feline pancreatic lipase polypeptides, splice variants, allelic variants, and fragments thereof. Isolated feline pancreatic lipase polypeptides, splice variants, allelic variants, and fragments thereof. Host cells comprising a vector containing the polynucleotide sequences and methods for expressing the polypeptides. The generation of monoclonal antibodies that specifically binds to the feline pancreatic lipase polypeptides, and cell lines secreting the monoclonal antibodies. Methods for determining the presence or amount of feline pancreatic lipase in a biological sample. The methods include using standards or calibrators of recombinant feline pancreatic lipase to quantify the lipase in a sample. Devices and kits for performing methods for detecting feline pancreatic lipase in biological samples.