Patent · US Active

Method of assaying target substance in sample, aptamer molecule method of constructing the same

US8143004B2 · kind B2 · utility

5Cited by

1References

8Claims

0Family size

Assignee

NATIONAL UNIVERSITY ORPORATION TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY · JP

Inventors

Kazunori Ikebukuro · Fuchu, JP
Koji Sode · Tokyo, JP

Key dates

Filing date	Sep 27, 2007
Grant date	Mar 27, 2012
Priority date	—
Expiry date	Feb 17, 2028

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/5308
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A method for measuring a test substance whereby the test substance in a sample can be specifically measured without using an antibody against the test substance; an aptamer molecule used therefor; and a method for creating the aptamer are disclosed. An aptamer capable of hybridizing with an oligonucleotide when it is bound to a test substance, but is incapable of hybridizing with the oligonucleotide when it is not bound to the test substance, is utilized. The aptamer is brought into contact with a sample, and the aptamer bound to the test substance is brought into contact with an immobilized oligonucleotide which hybridizes with the aptamer, to bind the aptamer to a solid phase, followed by measurement of the aptamer immobilized on the solid phase.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.