3D imaging of live cells with ultraviolet radiation
US8143600B2 · kind B2 · utility
17Cited by
84References
38Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 18, 2008 |
| Grant date | Mar 27, 2012 |
| Priority date | — |
| Expiry date | Jan 27, 2031 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/1787
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.