Microtitration plates comprising wells coated with a lipid substrate and a method of preparing
US8148096B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 2006 |
| Grant date | Apr 3, 2012 |
| Priority date | — |
| Expiry date | Feb 28, 2027 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2405/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for the detection and/or measurement in vitro of a lipase or phospholipase activity, including the addition of a sample likely to contain said lipase or phospholipase into the wells of microtitration plates coated with a layer of a lipid substrate which is able to be hydrolyzed by the lipase or phospholipase by releasing α-eleostearic acid, and the detection and/or the measurement of the lipase or phospholipase activity by UV spectrophotometry of the α-eleostearic acid released during the previous stage. The application of this method to the in vitro diagnosis of pathologies linked to an increase in the plasma lipase level.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.