Scaleable manufacturing process for cysteine endoprotease B, isoform 2

US8148105B2 · kind B2 · utility

Assignees

• The Board of Trustees of the Leland Stanford Junior University · US
• Alvine Pharmaceuticals, Inc. · US

Inventors

• Harmit Vora · South San Francisco, US
• James McIntire · Castro Valley, US
• Pawan Kumar · Norwalk, US
• Chaitan Khosla · Palo Alto, US

Key dates

• Filing date: Mar 14, 2008
• Grant date: Apr 3, 2012
• Expiry date: Mar 14, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/641
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods are provided for the production of gram to kilogram quantities of pro-EP-B2 (proenzyme form of EP-B2) in a lyophilized form. The methods include scalable fermentation, refolding and purification processes, which processes may be combined with lyophilization to yield a stable product.