Patent · US Expired

Method for the preparation of embryoid bodies (EBs) and uses thereof

US8148152B2 · kind B2 · utility

21Cited by

2References

48Claims

0Family size

Assignee

AXIOGENESIS AG · DE

Inventors

Eugen Kolossov
Ralf Kettenhofen · Bonn, DE
Isabella Kopp
Heribert Bohlen · Cologne, DE
Silke Schwengberg · St. Blasien, DE

Key dates

Filing date	Jul 8, 2004
Grant date	Apr 3, 2012
Priority date	—
Expiry date	Feb 9, 2025

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2510/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

Provided are means and methods for producing embryoid bodies (EBs) from multi- or pluripotent cells. In particular, a method of generating embryoid bodies (EBs) is described comprising agitation of a liquid suspension culture of multi- or pluripotent cells in a container until generation of cell aggregates, optionally diluting the suspension, and further agitation of the suspension until formation of EBs. Furthermore, the present invention relates to the use of the novel culturing method and EBs obtained thereby for a variety of applications including genomics, diagnostic assays, teratogenic/embryotoxicological and pharmacological assays as well as for the provision of tissue grafts.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.