System and methods for stretching polynucleotides
US8148159B2 · kind B2 · utility
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Key dates
| Filing date | Oct 5, 2007 |
| Grant date | Apr 3, 2012 |
| Priority date | — |
| Expiry date | Dec 12, 2028 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Apparatus and methods are described for achieving uniform stretching of polynucleotides in hybrid electrophoretic-gel, micro-constricting microfluidic channels. Polynucleotides in normally relaxed configurations are driven by an electric field along a microfluidic channel. The polynucleotides thread through a porous gel barrier formed in the channel and extend into a constriction where an electric field gradient exists. The combined action of the gel and field gradient acts to extend the polynucleotide configuration fully for direct linear analysis of the molecule.
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