Methods and means for removal of a selected DNA sequence
US8148607B2 · kind B2 · utility
2Cited by
4References
14Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 31, 2006 |
| Grant date | Apr 3, 2012 |
| Priority date | — |
| Expiry date | Mar 25, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8201
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. This method can be applied in a method for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.