Apparatus and method for dynamic cellular probing and diagnostics using holographic optical forcing array
US8149416B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Oct 30, 2008 |
| Grant date | Apr 3, 2012 |
| Priority date | — |
| Expiry date | Nov 2, 2029 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG03H2223/23
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention utilizes a holographic optical forcing array for dynamic cellular probing and diagnostics. A holographic optical trapping system generates optical forces on objects so that deformations thereof may be quantified. In one embodiment, digital holography is used to generate an interference pattern, and an analysis thereof determines the phase profile which yields a measurement of the objects' shape deformation using only one image. In another embodiment, phase-stepped holography allows the phase profile of an object to be measured using only one image, by using a holographic optical element to make phase-shifted replicas of the beam in space. In another embodiment, the optical forcing array applies optical forces to beads placed on the objects' surface, deforming the objects. The beads' position is determined by applying Mie theory, and analysis thereof yields the three dimensional position of the beads, and a measurement of the deformation displacement on the objects' surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.