Purification of proteins
US8163886B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Dec 20, 2007 |
| Grant date | Apr 24, 2012 |
| Priority date | — |
| Expiry date | Sep 1, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/36
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities (DNA, RNA, host cell protein, endotoxins, etc) in a cell broth and remains capable of binding to that entity even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered and further processed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.